Database : HANSEN Search on : MYCOBACTERIUM LEPRAE/FISIOL [Subject descriptor] References found : 100 [refine] Displaying: 1 .. 20   in format [Detailed]

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Id: 19737 Author: Tomioka, Haruaki; Yamada, Yoshitaka; Saito, Hajime; Jidoi, Joji. Title: Susceptibilities of Mycobacterium leprae and M. avium complex to the H202-Fe-mediated halogenation system supplemented with antimicrobial agents. Source: Int J Lep;57(3):628-632, sept. 1989. ^btab. Abstract: The susceptibilities of Mycobacterium leprae and M. avium complex (MAC) to the H2-O2-Fe-mediated halogenation system supplemented with antimicrobial agents were evaluated by fluorescein diacetate-ethidium bromide (FDA/EB) staining. In the case of M. leprae, the number of greenstained bacteria (intact cells) was reduced in the presence of the H2O2-Fe-mediated halogenation system supplemented with agents possessing antileprosy activity, such as rifampin, 4,4'-diaminodiphenylsulfone (dapsone), clofazimine, and ofloxacin. In the case of the MAC strain, although viable units of the organisms were reduced by the halogenation system alone, the number of greenstained cells in the FDA/EB stain was not reduced, even when the halogenation system was used in combination with ofloxacin. Because stainability of the cells is related to structural and functional intactness of the membrane, differences between M. leprae and the MAC strain imply possible differences in the rigidity of the cell membrane^ien. Descriptors: Mycobacterium leprae/imunol Mycobacterium avium/imunol Mycobacterium avium/fisiol Mycobacterium leprae/fisiol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1989/pdf/v57n3/v57n3a05.pdf / en Location: Br191.1

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Id: 19691 Author: Seo, Young- Hoon; Cho, Wook; Choi, Hae-Young; Hah, Yong-Ma; Cho, Sang-Nae. Title: Correspondence: Mycobacterium leprae in the Epidermis: Ultrastructural Study I. Source: Int. J. Lepr;63(1):101-103, 1995. ^bilus. Descriptors: Mycobacterium leprae/fisiol Epiderme/fisiopatol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1995/pdf/v63n1/v63n1cor04.pdf / en Location: BR191.1

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Id: 19690 Author: Sticht- Groh, V; Bretzel, G; Harmsen, D; Heesemann, J; Ishaque, M. Title: Correspondence: An Unusual Spreading Phenomenon in an Atificial Medium Inoculated with M. leprae. Source: Int. J. Lepr;63(1):100-101, 1995. ^bilus. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/fisiol Location: BR191.1

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Id: 19689 Author: Mostafa, Hamdy Mahfous; Kazda, Jindrich; Irgens, Lorents; Luesse, Hans Gerd. Title: Correspondence: Acid- Fast Bacilli from Former Leprosy Regions in Coastal Norway Showing PCR Positivity for Mycobacterium leprae. Source: Int. J. Lepr;63(1):97-99, 1995. ^bilus, ^btab. Descriptors: Mycobacterium leprae/fisiol Reação em Cadeia da Polimerase/métodos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1995/pdf/v63n1/v63n1cor02.pdf / en Location: BR191.1

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Id: 19686 Author: anon. Title: Editorial: Mycobacterium leprae Iron Nutrition: Bacterioferritin, Mycobactin, Exochelin and Intracellular Growth. Source: Int. J. Lepr;63(1):86-91, 1995. . Descriptors: Mycobacterium leprae/cresc Mycobacterium leprae/fisiol Bacteriocinas/anal Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1995/pdf/v63n1/v63n1edt.pdf / en Location: BR191.1

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Id: 19661 Author: Roche, Paul W; Failbus, Sarah S; Britton, Warwick J; Cole, Robert. Title: Rapid method for diagnosis of leprosy by measurements of antibodies to the M. leprae 35-kDa protein: comparison with PGL-1 antibodies detected by ELISA and "Dipstick" methods. Source: Int. J. Lepr;67(3):279-286, Sept., 1999. ilus, graf. Abstract: A new rapid immuno-chromatographic test card for the detection of antibodies to the Mycobacterium leprae 35-kD protein is described. The new assay is compared in the same group of subjects with a direct enzyme ELISA method for 35-kD antibodies and with assays for anti-phenolic glycolipid-I (PGL-I) antibodies using a standard ELISA as well as the recently described [quot ]dipstick[quot ] method. Good concordance was found between the rapid methods and the corresponding ELISA methods. The detection of untreated paucibacillary leprosy by the 35-kD test card was 59% compared with 27% for the PGL-I dipstick; however, the specificity for the 35-kD test card was 90% compared with 100% for the PGL-I dipstick in an endemic population. The potential application of these new, rapid serologic methods for the diagnosis of leprosy under field conditions is discussed. (AU)^ien. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/fisiol Anexina A3/imunol ELISA/métodos Limits: Humanos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n3/v67n3a07.pdf / en Location: BR191.1

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Id: 19646 Author: Negesse, Yohannes. Title: Staging nerve involvement in M. Leprae infection. Source: Int. J. Lepr;67(2):167-168, Jun., 1999. . Descriptors: Mycobacterium leprae/imunol Mycobacterium leprae/fisiol Doenças do Sistema Nervoso/fisiopatol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n2/v67n2cor03.pdf / en Location: BR191.1

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Id: 19631 Author: Storrs, Eleanor E. Title: A lost Talisman: catastrophic decline in yields of leprosy bacilli from armadillos used for vaccine production. Source: Int. J. Lepr;67(1):67-70, Mar., 1999. tab. Descriptors: Mycobacterium leprae/imunol Mycobacterium leprae/fisiol Vacinas/imunol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n1/v67n1cor03.pdf / en Location: BR191.1

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Id: 19625 Author: Shimizu, Toshiaki; Maw, Win Win; Tomioka, Haruaki. Title: Roles of tumor necrosis factor-alpha and transforming growth factor-beta in regulating intercellular adhesion molecule-1 expression on murine peritoneal macrophages infected with M. Leprae. Source: Int. J. Lepr;67(1):36-45, Mar., 1999. tab, graf. Abstract: Profiles of intercellular adhesion molecule-1 (ICAM-1) expression on murine peritoneal macrophages (M phi s) infected with Mycobacterium leprae during cultivation were examined with special reference to the regulatory effects of tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta (TGF-beta). When M phi s were infected with M. leprae or stimulated with heat-killed M. leprae at day 0, their ICAM-1 expression, measured in terms of the ratio of M phi s positively stained with anti-ICAM-1 antibody (Ab), rapidly increased, peaking during days 1 to 3 and thereafter fell, returning to the normal level by day 7. The addition of TNF-alpha or anti-TGF-beta Ab inhibited the middle phase (day 7) downregulation of M phi ICAM-1 expression, although the late-phase (day 14) downregulation of ICAM-1 was not prevented by them. M. leprae-infected M phi s released small amounts of TNF-alpha and significant amounts of TGF-beta into the culture medium. This may indicate that M. leprae-infected M phi s produced the majority of TNF-alpha in a membrane-bound form. Alternatively, endogenous TNF-alpha might upregulate M phi ICAM-1 expression even at very low concentrations. In any case, these findings indicate the central roles of TNF-alpha and TGF-beta in the early phase upregulation and the middle-to-late phase downregulation, respectively, of ICAM-1 expression by M. leprae-infected M phi s. (AU)^ien. Descriptors: Fator de Necrose Tumoral alfa/imunol Fator de Necrose Tumoral alfa/fisiol Macrófagos/imunol Macrófagos/microbiol Mycobacterium leprae/imunol Mycobacterium leprae/fisiol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n1/v67n1a06.pdf / en Location: BR191.1

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Id: 19601 Author: Bapat, CV. Title: Cultivation of mycobacterium leprae: a new approach. Source: Int. J. Lep;57(4):874-879, dec. 1989. ^btab. Descriptors: Mycobacterium leprae/imunol Mycobacterium leprae/fisiol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1989/pdf/v57n4/v57n4cor06.pdf / en Location: Br191.1

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Id: 19592 Author: Nomaguchi, Hiroko; Matsuoka, Masanori; Kohsaka, Kenji; Nakata, Atsuo; Ito, Tonetaro. Title: Overproduction, affinity purification and characterization of 65-kDA protein of mycobacterium leprae in Escharichia coli. Source: Int. J. Lep;57(4):817-824, dec. 1989. . Abstract: The 65-kDa protein of Mycobacterium leprae was produced in an Escherichia coli strain carrying a plasmid harboring the recloned gene coding for the protein. The protein was purified through affinity chromatography prepared with the IgG fraction of a monoclonal antibody which was prepared against the 65-kDa protein. The purified 65-kDa protein also reacted immunologically with the monoclonal antibody IIIE9, which recognizes the epitope for M. leprae, prepared by Buchanan, et al. BALB/c mice were inoculated with M. leprae and 4 months later were skin tested with the purified 65-kDa protein. Gross changes were observed at the skin-test site. The role of the protein in protective immunity against M. leprae foot pad infection in mice was also studied. Descriptors: Mycobacterium leprae/imunol Mycobacterium leprae/fisiol Escherichia coli/imunol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1989/pdf/v57n4/v57n4a10.pdf / en Location: Br191.1

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Id: 19529 Author: Adams, Linda B; Soileau, Nashone A; Battista, John R; Krahenbuhl, James L. Title: Inhibition of metabolism and growth of mycobacterium leprae by Gamma irradiation. Source: Int. J. Lepr;68(1):1-10, Mar., 2000. ilus, graf. Abstract: Mycobacterium leprae is uncultivable on artificial medium, but viability can be maintained without multiplication for a limited time in vitro. In this study, we evaluated gamma-irradiation (gamma-irr) as a means to kill this slowly growing organism. Freshly harvested, viable, athymic, nu/nu mouse-derived M. leprae were exposed to varying doses of gamma-irr from a 60Co source. Two indicators of bacterial viability were determined: metabolism, measured by oxidation of 14C-palmitic acid to 14CO2 in the BACTEC 460 system, and multiplication, measured by titration in the mouse foot pad. gamma-Irr of both M. leprae and M. lufu, a cultivable control mycobacterium, resulted in a dose-dependent inhibition of viability. gamma-Irr of up to 10(3) rad had little effect on the metabolic activity of either organism. For M. leprae, 10(4)-10(5) rad caused an intermediate inhibitory effect; whereas 10(6) rad yielded almost total inhibition. In the mouse foot pad assay, up to 10(4) rad had little effect on M. leprae growth; however, 10(5) rad resulted in at least a 2-log reduction in the number of bacilli recovered and no M. leprae growth was measurable after exposure to 10(6) rad. With M. lufu, 10(5) rad inhibited metabolic activity by 99% and caused > or = 2-log reduction in the number of colony forming units (CFU). No CFU of M. lufu were recovered after exposure to 10(6) rad. Scanning electron microscopy revealed the presence of some aberrant protrusions on the cell surface of lethally irradiated M. leprae; whereas boiling and autoclaving caused obvious morphological denaturation. These data suggest that gamma-irr is an effective way to kill M. leprae without causing extensive damage to the cell architecture. Killing M. leprae by gamma-irr may be preferable when comparing cellular responses to live versus dead bacilli in vitro and in vivo. (AU)^ien. Descriptors: Mycobacterium leprae/metab Mycobacterium leprae/fisiol Usos da Radiação Raios gama/uso terap Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2000/pdf/v68n1/v68n1a01.pdf / en Location: BR191.1

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Id: 19421 Author: Parkash, Om. Title: A study on the reproducibility of two serological assays for detection of Mycobacterium leprae infection. Source: Int. J. Lepr;69(1):46-48, Mar., 2001. tab. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/imunol Mycobacterium leprae/fisiol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n1/v69n1cor06.pdf / en Location: BR191.1

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Id: 19414 Author: Zumarraga, Martin Jose; Resoagli, Edmundo Hector; Civuta, Maria Helena; Martinez, Anibal Ramon; Rott, Maria Izabel Ortiz de; Millan, Sonnia Gracia de; Caimi, Karina; Gioffre, Andrea; Alito, Alicia; Bigi, Fabiana; Cataldi, Angel Adrian; Romano, Maria Isabel. Title: PCR-Restriction fragment length polymorphism analysis (PRA) of mycobacterium leprae from human lepromas and from a natural case of an armadillo of Corrientes, Argentina. Source: Int. J. Lepr;69(1):21-25, Mar., 2001. ilus. Abstract: Polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis (PRA) which relies on the amplification of a 439-bp portion of the hsp65 gene present in all mycobacteria, followed by two distinct digestions (with BstEII and HaeIII) of the PCR product, offers a rapid and easy alternative that allows identification of the species without the need for specialized equipment. Wild leprosy in the nine-banded armadillo (Dasypus novemcinctus) is characterized by the presence of multiple bacilli in internal organs such as lymph nodes, spleen and liver, as well as in nerves and skin. We could observe this in 9 out of 132 animals captured in Corrientes, Argentina, an area endemic for leprosy in humans. Mycobacterium leprae were recognized in those naturally infected animals through different techniques. Three samples of extracted DNA of the mycobacteria present in the spleen, liver and popliteal lymph node of a naturally infected animal during the Experimental Program in Armadillo (PEA) and three samples of human lepromas were processed by PRA. The patterns of the six samples analyzed were identical and were characteristic of M. leprae. These studies, made for the first time in Argentina, corroborate the initial discoveries in South America made by our investigative group on the detection of armadillos naturally infected with the Hansen bacillus.(AU)^ien. Descriptors: Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos Mycobacterium leprae/genet Mycobacterium leprae/imunol Mycobacterium leprae/fisiol Limits: Animais Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n1/v69n1a03.pdf / en Location: BR191.1

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Id: 19413 Author: Nakamura, Masahiro; Matsuoka, Masanori. Title: Limited ATP generation in cells of mycobacterium leprae thai-53 strain in enriched kirchner liquid medium containing adenosine. Source: Int. J. Lepr;69(1):13-20, Mar., 2001. ilus, tab, graf. Abstract: The ATP generation in cells of Mycobacterium leprae Thai-53 strain takes place in vitro when the cells are cultivated in Kirchner liquid medium, pH 7.0, enriched with egg-yolk solution, pyruvate, transferrin, and adenosine at 30 degrees C. Among the supplements, adenosine was key and critical for the ATP generation. The optimal concentration of adenosine was 50 micrograms/ml of the medium. ATP generation, however, was limited; the rates of increase in ATP content extracted from the cells were approximately two- to threefold compared to that of the starting samples, and the increase reached a maximum at 4 or 6 weeks after incubation. No significant ATP generation in M. leprae cells was demonstrated in medium at pH 6.2 or pH 6.6, in the original Kirchner medium with or without adenosine, or when cultured at 37 degrees C, or when containing an antileprosy drug. No detectable increase in the number of M. leprae cells was observed with the increase in intracellular ATP content and DNA replication. No effect was seen with renewal of the cultured medium by freshly prepared medium at 6 weeks' cultivation on the progressive ATP generation in M. leprae. (AU)^ien. Descriptors: Trifosfato de Adenosina/imunol Trifosfato de Adenosina/fisiol Adenosina/imunol Mycobacterium leprae/imunol Mycobacterium leprae/fisiol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n1/v69n1a02.pdf / en Location: BR191.1

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Id: 19378 Author: Sarkar, Arun De; Kaur, Inderjeet; Radotra, Bishan Dass; Kumar, Bhushan. Title: Impact of combined Mycobacterium w vaccine and 1 year of MDT on multibacillary leprosy patients. Source: Int. J. Lepr;69(3):187-194, Sept., 2001. ilus, tab. Abstract: A total of 20 bacteriologically positive multibacillary (MB) leprosy patients older than 18 years of age with a bacterial index (BI) of 2+ or greater were given standard World Health Organization multiple drug therapy (MDT-MB) for 12 consecutive months plus four intradermal doses of Mycobacterium w vaccine at 3 monthly intervals (Study group). Twenty age-matched MB patients were given WHO/MDT alone (Control group). The patients of both groups were followed up for 1 year. Improvements in the patients were periodically monitored by clinical (Ramu's score), bacteriological (SSS), histopathological (skin biopsy) and immunological (lepromin conversion) parameters. Study group patients showed more significant improvements in all parameters except for lepromin conversion compared to patients in the Control group. The incidence of type 1 reaction was more in the Study group (30% vs 10%), while the incidence of type 2 reaction was more in the Control group (25% vs 15%). Neuritis associated with reactions was seen more often in the Control group compared to the Study group (20% vs 10%). The addition of Mycobacterium w vaccine as an adjunct to the 1-year WHO/MDT regimen appears to be significantly more beneficial in MB leprosy patients with a high initial BI compared to WHO/MDT given alone. Studies on larger numbers of patients with extended follow up will be in order. (AU)^ien. Descriptors: Hanseníase/quimioter Hanseníase/imunol Hanseníase/fisiopatol Mycobacterium leprae/genet Mycobacterium leprae/imunol Mycobacterium leprae/fisiol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n3/v69n3a02.pdf / en Location: BR191.1

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Id: 19359 Author: Jadhav, Rupendra S; Macdonald, Murdo; Bjune, G; Oskam, L. Title: Simplified PCR detection method for nasal Mycobacterium leprae. Source: Int. J. Lepr;69(4):299-307, Dec., 2001. ilus, tab. Abstract: We report here a simplified method for the detection of nasal carriage of Mycobacterium leprae. DNA extracted from nasal swabs was analyzed by PCR, and M. leprae specific amplicons detected by means of a novel peptide-nucleic-acid-ELISA (PNA-ELISA) method. Parameters for the method were established using swabs taken from untreated lepromatous leprosy patients. We have developed this method to study nasal carriage in endemic populations. However, due to the sensitivity of PCR based techniques, we wished to assess the possibility of false positive samples arising in our method. We therefore examined samples taken from individuals in Norway, a country non-endemic for leprosy, using our technique. A total of 219 nasal swabs were collected and tested in our laboratory in London. All of these were found to be negative by our criteria. In order to corroborate our results, and also to assess the specificity of the method, a small number of these samples were randomly selected, and a known amount of M. leprae DNA added to them. All 219 samples were then retested using the same techniques under [quot ]double blind[quot ] conditions in our laboratory in India. All of the samples to which M. leprae DNA had been added were successfully identified by this method whereas all other swabs were negative. Taken together, these results suggest that the technique described here is simple, sensitive, and specific for use in large-scale epidemiological studies. This study, part of the larger MILEP 2 study, represents the first use of a PNA-PCR method for an epidemiological study of infection. The method using PNA-ELISA is significantly simpler and more rapid than gel based detection methods. The supply of laboratory consumables and overall detection procedure were simplified and standardized by use of PCR Ready-to-Go beads.(AU)^ien. Descriptors: Reação em Cadeia da Polimerase/métodos Mycobacterium leprae/genet Mycobacterium leprae/imunol Mycobacterium leprae/fisiol Limits: Humanos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n4/v69n4a01.pdf / en Location: BR191.1

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Id: 19329 Author: Slayden, Richard A. Title: Reports on current issues and workshops: report of the workshop on genome, transcriptome and proteome of M. leprae: aplication to basic and clinical questions. Source: Int. J. Lepr;70(4):329-330, Dec., 2002. . Conference: Present in: International Leprosy Congress, 16, Salvador, 4-9 Aug., 2002. Descriptors: Mycobacterium leprae/citol Mycobacterium leprae/genet Mycobacterium leprae/imunol Mycobacterium leprae/isol Mycobacterium leprae/fisiol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2002/pdf/v70n4/v70n4repcur01.pdf / en Location: BR191.1

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Id: 19307 Author: Ebenezer, Gigi J; Arumugam, Shantha; Job, Charles K. Title: Dosage and site of entry influence growth and ddissemination of mycobacterium leprae in T900r mice. Source: Int. J. Lepr;70(4):245-249, Dec., 2002. ilus, tab. Abstract: The role of dosage of Mycobacterium leprae and the environment of the inoculated site, in producing leprosy lesions in immunologically-suppressed, highly-susceptible T900r mice, was investigated. Various doses of M. leprae, i.e., 10(7), 10(6), 10(5), 10(4), were inoculated into both flanks and footpads of two different groups of mice. The sites of inoculation were biopsied for histopathological examination and for M. leprae counts at the end of 6, 8 and 12 months. M. leprae multiplied at the infected site and disseminated [figure: see text] to other parts of the body at all concentrations in the mice that were infected in the footpad with a temperature of 31 degrees C. In animals inoculated at the flanks with a temperature of 37 degrees C, multiplication was recorded only when the dosage of M. leprae was high and there was no dissemination of the organism in any of them. The temperature at the site of entry and the dose of infecting M. leprae may play an important role in the development of leprosy in susceptible individuals exposed to M. leprae. (AU)^ien. Descriptors: Hanseníase/imunol Mycobacterium leprae/imunol Mycobacterium leprae/fisiol Limits: Animais Camundongos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2002/pdf/v70n4/v70n4a01.pdf / en Location: BR191.1

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Id: 19248 Author: Shetty, Vanaja Prabhakar; Antia, Noshir Hormusji. Title: Light and ultrastructural study of sciatic nerve lesions induced using intraneural injection of viable mycobacterium leprae in normal and immunosuppressed swiss white mice. Source: Int. J. Lepr;70(1):25-33, Mar.,2002. ilus, tab. Abstract: Freshly harvested M. leprae were microinjected into the sciatic nerves of nonimmunosuppressed (non-TR) and immunosuppressed (TR) mice using the technique described by Wisniewski and Bloom. The lesions thus induced, on bypassing the blood-nerve barrier, were biopsied at regular intervals beginning 24 hr and followed up to one year. The fate of M. leprae and the ensuing inflammation and nerve damage were studied using light and electron microscopy. The lesions in both non-TR and TR mice at 24 hr showed an influx of polymorphonuclear leukocytes and an increase in mast cells. The influx and peaking of lymphocytes were delayed by two weeks and 6 weeks, respectively, in TR mice, but the density of lymphocytes at the peak intervals was comparable in both. The plasma cells denoting the humoral response were seen in both, but there was a delay of 3 weeks in non-TR mice. The lesions in non-TR mice showed differentiation of macrophages into epithelioid cells and the formation of giant cells depicting borderline tuberculoid leprosy (BT), Whereas in TR mice, the macrophages showed foamy cytoplasmic changes depicting borderline lepromatous leprosy (BL). Other significant observations common to both non-TR and TR mice were: a) The lesions remained highly localized and showed signs of regression at the 6th and the 12th month intervals. b) The characteristic segmental demyelination and some attempt at remyelination were seen at the site. c) The influx of lymphocytes concorded well with demyelination. d) Bacteria were only seen in the macrophages and never in the Schwann cells or endothelial cells. e) Bacteria persisted in the macrophages, but appeared progressively degenerate at the 6th and 12th post-inoculation months, suggesting loss of viability. The study shows that there was a very effective containment of the infection and that the Schwann cells were resistant to M. leprae infection in the neural milieu. Nerve damage and Schwann cell bacillation do not go hand-in-hand. (AU)^ien. Descriptors: Nervo Ciático/anorm Nervo Ciático/les Nervo Ciático/fisiopatol Mycobacterium leprae/fisiol Mycobacterium leprae/ultraest Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2002/pdf/v70n1/v70n1a04.pdf / en Location: BR191.1

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